SEROLOGICAL DIAGNOSIS OF BOVINE CORONAVIRUS IN CALVES USING A DEVELOPMENT NATIVE ELISA PREPARATION

Bovine coronavirus (BCoV) infection has found worldwide among cattle of all ages, causing diarrhea in newborn calves, winter dysentery adult and respiratory tract diseases results major economic losses dairy herds. The objective this study was to establish an ELISA kits as patent prepared Lab. with assessment its applicability the field samples. Sixty-five pneumonic calves 2- 4-month-old, used detect BCoV antibodies serum infected where it assayed against by using plates SNT. were positive for antibody 72.3% 53.9% SNT respectively. showed high sensitivity over SNT, sensitivity, specificity correlation 91.4%, 50% respectively, there is a between both tests. test been successfully longitudinal studies on virus dynamics evaluation management routines. In conclusion more sensitive, accurate specific assay than detection antibodies, addition saving time commercial cost.